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File:A-Novel-Approach-for-Purification-and-Selective-Capture-of-Membrane-Vesicles-of-the-pone.0095137.s004.ogv

A-Novel-Approach-for-Purification-and-Selective-Capture-of-Membrane-Vesicles-of-the-pone.0095137.s004.ogv (Ogg Theora video file, length 6.0 s, 512 × 512 pixels, 3.54 Mbps, file size: 2.53 MB)


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Summary

Description
English: Morphological changes and movement of cells in the presence of MVs. Time-lapse observation of a monolayer of Ca9.22 cells was monitored by an optical microscope (Olympus) equipped with a CCD camera (Andor) in the presence of highly purified MVs isolated by the SB technique. The protein concentration of MVs used in the assay was 20 µg/ml. Images were taken at 30 sec/frame for 30 min immediately after addition of MVs suspended in Eagle’s balanced salt solution (EBSS). The constructed time-lapse images are shown at 100 msec/frame (300 times the original speed). The width of the image is 170 µm.
Date
Source Video S1 from Nakao R, Kikushima K, Higuchi H, Obana N, Nomura N, Bai D, Ohnishi M, Senpuku H (2014). "A Novel Approach for Purification and Selective Capture of Membrane Vesicles of the Periodontopathic Bacterium, Porphyromonas gingivalis: Membrane Vesicles Bind to Magnetic Beads Coated with Epoxy Groups in a Noncovalent, Species-Specific Manner". PLOS ONE. DOI:10.1371/journal.pone.0095137. PMID 24830438. PMC: 4022494.
Author Nakao R, Kikushima K, Higuchi H, Obana N, Nomura N, Bai D, Ohnishi M, Senpuku H
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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a5184460c7f9042992574bb28c5698282b1ef24a

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512 pixel

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current21:03, 22 May 20146.0 s, 512 × 512 (2.53 MB)Open Access Media Importer BotAutomatically uploaded media file from Open Access source. Please report problems or suggestions here.

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