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File:Hela 4xegfp SPIM.ogv

Hela_4xegfp_SPIM.ogv ‎(Ogg Theora video file, length 25 s, 384 × 350 pixels, 221 kbps, file size: 685 KB)


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Summary

Description
English: volumetric reconstruction (with FIJI) of a z-stack taken on cells expressing EGFP-tetramer molecules imaged with a single plane illumination microscope (SPIM). The cells were adherent to a small peace of coverslip which was moved through the SPIM image plane. Excitation wavelength: 491nm, detection objective: 60x, NA1, voxel size: 0.4x0.4x0.4µm³, EMCCD camera, lightsheet 1/e²-diameter ~2.4µm The images were acquired by Jan Krieger (Jkrieger (talk) 16:42, 14 September 2012 (UTC)) in the group Biophysics of Macromolecules (Langowski lab) at the German Cancer Research Center (DKFZ) in heidelberg.
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Source Own work
Author Jkrieger

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This file is licensed under the Creative Commons Attribution-Share Alike 3.0 Unported license.
Attribution: Jan Krieger / Deutsches Krebsforschungszentrum (AG Biophysik der Makromoleküle, Prof. Jörg Langowski)
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14 September 2012

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Date/TimeThumbnailDimensionsUserComment
current16:42, 14 September 201225 s, 384 × 350 (685 KB)Jkrieger{{Information |Description ={{en|1=volumetric reconstruction (with FIJI) of a z-stack taken on en:HeLa cells expressing EGFP-tetramer molecules imaged with a single plane illumination microscope (SPIM). The cells were adherent to a sm...

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