File:Mitigating-Phototoxicity-during-Multiphoton-Microscopy-of-Live-Drosophila-Embryos-in-the-1.0-1.2-µm-pone.0104250.s004.ogv
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Summary
| Description |
English: 3D imaging of a sna- (top) and a wild-type (bottom) Drosophila embryo with THG during gastrulation (stages 5–8). Imaging conditions: 750 nm per pixel lateral sampling, 3 µm per pixel axial sampling, 3 µs per pixel, 30 planes by stack, 40 s per stack, continuous imaging, 1180 nm excitation wavelength, 100 fs pulses. Such dataset permits a direct comparison of morphogenetic movements in wild-type and mutant embryos for which fluorescent strains are often lacking. See also figure 5. |
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| Source | Video S4 from Debarre D, Olivier N, Supatto W, Beaurepaire E (2014). "Mitigating Phototoxicity during Multiphoton Microscopy of Live Drosophila Embryos in the 1.0-1.2 µm Wavelength Range". PLOS ONE. DOI:10.1371/journal.pone.0104250. PMID 25111506. PMC: 4128758. | ||
| Author | Debarre D, Olivier N, Supatto W, Beaurepaire E | ||
| Permission (Reusing this file) |
  This file is licensed under the Creative Commons Attribution 4.0 International license.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 02:31, 15 August 2014 | 4.3 s, 748 × 700 (3.04 MB) | Open Access Media Importer Bot | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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