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Summary

Description
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In microarrays, the ds-cDNA is fragmented and fluorescently labelled (orange). The labelled fragments bind to an ordered array of complementary oligonucleotides and measurement of fluorescent intensity across the array indicates the abundance of a predetermined set of sequences. These sequences are typically specifically chosen to report on genes of interest within the organism’s genome.[1]
Date
Source Own work
Author Thomas Shafee
Other versions

Also in series:

  • RNA Microarray
    RNA Microarray
  • RNA-Seq
    RNA-Seq
  • SAGE
    SAGE

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  1. ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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DNA microarray

microarray

inception

5 October 2016

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Date/TimeThumbnailDimensionsUserComment
current12:22, 13 February 2017Thumbnail for version as of 12:22, 13 February 20171,020 × 858 (67 KB)Evolution and evolvabilitytext as paths to avoid artefacts
08:40, 10 February 2017Thumbnail for version as of 08:40, 10 February 20171,020 × 858 (66 KB)Evolution and evolvabilityReverted to version as of 05:08, 5 October 2016 (UTC)
08:40, 10 February 2017Thumbnail for version as of 08:40, 10 February 20171,020 × 803 (87 KB)Evolution and evolvabilityreverse order of fragmentation and reverse-transcription.
05:08, 5 October 2016Thumbnail for version as of 05:08, 5 October 20161,020 × 858 (66 KB)Evolution and evolvabilityUser created page with UploadWizard

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