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File:Widefield-Two-Photon-Excitation-without-Scanning-Live-Cell-Microscopy-with-High-Time-Resolution-and-pone.0147115.s005.ogv

Widefield-Two-Photon-Excitation-without-Scanning-Live-Cell-Microscopy-with-High-Time-Resolution-and-pone.0147115.s005.ogv ‎(Ogg Theora video file, length 10 s, 356 × 424 pixels, 2.08 Mbps, file size: 2.51 MB)


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English: Localisation of changes in fluorescence intensity. Widefield two-photon excitation microscopy shows Ca2+ transients in live neuronal cell bodies loaded with Fluo-4 AM at a frame rate of 100 Hz over a duration of 10 seconds. Three regions of interest (white, red and green) are shown in three adjacent cell bodies to demonstrate not only the rate of image capture but to confirm that the measured change in fluorescence signal intensity with time was not a consequence of fluctuations in laser power or camera instability, but arose from localised changes in intracellular Ca2+ concentration. Scale bar = 15 μm.
Date
Source S2 Video from Amor R, McDonald A, Trägårdh J, Robb G, Wilson L, Abdul Rahman N, Dempster J, Amos W, Bushell T, McConnell G (2016). "Widefield Two-Photon Excitation without Scanning: Live Cell Microscopy with High Time Resolution and Low Photo-Bleaching". PLOS ONE. DOI:10.1371/journal.pone.0147115. PMID 26824845. PMC: 4732674.
Author Amor R, McDonald A, Trägårdh J, Robb G, Wilson L, Abdul Rahman N, Dempster J, Amos W, Bushell T, McConnell G
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Date/TimeThumbnailDimensionsUserComment
current10:49, 7 February 201610 s, 356 × 424 (2.51 MB)Open Access Media Importer BotAutomatically uploaded media file from Open Access source. Please report problems or suggestions here.

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